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Phlebotomy Certification Nha Ascp

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200 cards · ~100 min · Advanced · Updated

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What is the correct order of draw for venipuncture (ETS)?

1. Blood cultures (yellow SPS)
2. Light blue (sodium citrate)
3. Serum tubes (red/gold, SST)
4. Green (heparin)
5. Lavender (EDTA)
6. Gray (sodium fluoride)

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Q · 1 of 200

What is the correct order of draw for venipuncture (ETS)?

Q · 2 of 200

Why must the order of draw be followed?

Q · 3 of 200

Which tube is drawn first in the order of draw?

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What is the correct order of draw for venipuncture (ETS)?

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1. Blood cultures (yellow SPS)
2. Light blue (sodium citrate)
3. Serum tubes (red/gold, SST)
4. Green (heparin)
5. Lavender (EDTA)
6. Gray (sodium fluoride)

Why must the order of draw be followed?

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To prevent cross-contamination of additives between tubes, which can alter test results.

Which tube is drawn first in the order of draw?

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Blood culture bottles/tubes (yellow SPS) to maintain sterility.

Which tube is drawn immediately after blood cultures?

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The light blue sodium citrate tube (coagulation studies).

Why is the light blue coagulation tube drawn early in the order of draw?

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To minimize contamination by tissue thromboplastin and other additives that could affect clotting results.

Where do serum separator tubes (SST, gold/tiger top) fall in the order of draw?

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After light blue and before tubes containing anticoagulants like heparin, EDTA, and fluoride.

Where does the green top (heparin) tube fall in the order of draw?

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After serum tubes and before lavender (EDTA) and gray (fluoride) tubes.

Where does the lavender top (EDTA) tube fall in the order of draw?

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After green (heparin) and before gray (sodium fluoride).

Which tube is drawn last in the standard order of draw?

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The gray top tube (sodium fluoride/potassium oxalate).

What is the order of draw when using a winged (butterfly) collection set for a coagulation tube first?

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A discard tube (non-additive or another citrate tube) is drawn first to clear air from the tubing before the light blue tube.

Does the order of draw differ for a syringe draw compared to a tube holder draw?

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No — the same CLSI order of draw sequence applies when filling tubes from a syringe.

What mnemonic is commonly used to remember the order of draw?

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"Stop Light Red Green Yellow Gray" for Sterile (blood culture), Light blue, Red/SST, Green, Yellow/Lavender, Gray.

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